Infinite® F500 - Features
High measurement speed and robotic compatibility |
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| The Infinite F500 filter-based microplate reader is capable of reading 6 to 384-well plates, including standard, half-area and low volume 384-well plates. All top-reading modes can be used for 1536-well microplates (optional) and, by selecting the high speed »on-the-fly« mode, a 1536-well plate can be read in less than 30 seconds (please refer to typical performance values). The Infinite F500 can also be easily integrated into the Freedom EVO® series of automated platforms. |
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Programmable filter slides |
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The Infinite F500 filter-based microplate reader includes programmable filter slides with electronic chips; a great tool for saving certain filter characteristics to support the filter definition of the corresponding filter
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1 The mirror carrier can be extended with a custom dichroic mirror; 2 Exchangeable filters can be easily fitted into the excitation and emission filter slides; 3 Separate excitation and emission filter slides with 6 filter positions each ; 4 Microchip on filter slide to facilitate filter definition/recognition; 5 Filter assembly tool |
Infinite® F500 - Applications
The optimal filter based microplate reader for biopharmaceutical, life science and research laboratories
The fast and sensitive Infinite F500 filter-based microplate reader is ideal for a broad range of applications, in particular ratiometric assays and other technologies routinely used within assay development, screening and ADMET laboratories, research institutes and the Life Sciences industry. It is capable of reading 6 to 1536-well microplates, including standard and low-volume plates.
The Infinite F500 microplate reader supports many different applications and assay technologies, such as:
- TR-FRET-based assays: e.g. HTRF® (Cisbio bioassays, France), LanthaScreen® (Invitrogen).
- FRET-based assays: e.g. Z`-LYTE® (Invitrogen) to study cellular protein kinases, GeneBLAzer® (Invitrogen) Reporter Gene assays.
- Glow luminescence: cell-based & reporter gene assays
- Flash luminescence: e.g. dual luciferase assay (DLR®) and various CA2+ detection assays if optional injectors are installed
- Fluorescence polarization: e.g. Predictor™ hERG Fluorescence Polarization Assay (Invitrogen), PolarScreen™ Glucocorticoid Receptor Competitor Assay Kit, Red (Invitrogen), PolarScreen™ Glucocorticoid Receptor Competitor Assay Kit, Green (Invitrogen), Transcreener® ADP Assay (BellBrook Labs)
The Infinite F500 has been validated by Invitrogen for the following Invitrogen assays:
If you are interested in more details on the applications for the Infinite F500 microplate reader, please take a look at the related literature.
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The Infinite F500 has been granted the LanthaScreen® Certified Plus Status by Invitrogen
PolarScreen and Predictor are trademarks of Invitrogen Corporation, Carlsbad, USA.
LanthaScreen, Adapta, GeneBLAzer, Omnia and Z’-LYTE are registered trademarks of Invitrogen Corporation, Carlsbad, USA. |
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The Infinite F500 has been successfully validated and certified for HTRF® by CisBio bioassays.
HTRF® is a registered trademark of Cisbio international, France. The fluorescence ratio associated with the HTRF® readout is a correction method developed by Cisbio international and covered by the US patent 5,527,684 and its foreign equivalents, for which Cisbio international has granted a license to Tecan Group Ltd. Its application is strictly limited to the use of HTRF® reagents and technology, excluding any other TR-FRET technologies. |
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The Infinite F500 has been successfully Transcreener® Far Red FP and Transcreener® FI validated by BellBrook Labs, LLC, USA and meets the requirements for BellBrook Labs’ Instrument Validation Program.
Transcreener® HTS Assay Platform is a patented technology of BellBrook Labs, LLC USA. Transcreener® is a registered trademark of BellBrook Labs, LLC USA. |
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The Infinite F500 has passed the DLReady™ validation at Promega and is certified as DLReady™.
DLReady™ is a trademark of Promega Corporation, USA
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Infinite® F500 - Typical performance values
| Light sources |
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High energy xenon flash lamp |
| Wavelength selection |
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Filter-based |
| Wavelength range |
| Absorbance |
230 – 1,000 nm |
| Fluorescence |
Ex: 230 – 900 nm; Em: 280 – 900 nm |
| Fluorescence polarization |
Ex: 300 – 750 nm; Em: 330 – 750 nm |
| Detectors |
| Absorbance |
UV Silicon photodiode |
| Fluorescence |
Extended wavelength (UV and far red) ;
Low dark current PMT |
| Luminescence |
Photon counting; Low dark current PMT |
| Plate formats |
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6 – 1,536-well plates, NanoQuant Plate |
| Temperature control |
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Ambient +4 to 42 °C |
| Shaking |
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Linear, orbital with variable amplitudes |
| Fluorescence sensitivity values |
| Fluorescence top |
10 amol/well fluorescein
(Greiner® 384-well low volume black plate; 10 µl) |
| Fluorescence bottom |
0.4 fmol/well fluorescein
(Greiner 96-well SensoPlateTM; 200 µl) |
| TRF |
0.5 amol/well europium
(Greiner 384-well low volume white plate; 10 µl) |
| FP |
< 2 mP standard deviation @ 1 nM fluorescein |
| Luminescence sensitivity values |
| Glow luminescence* |
225 amol/well ATP
(Greiner 384-well low volume white plate; 25 µl) |
| Flash luminescence** |
12 amol/well ATP
(Greiner 384-well white plate; 55 µl) |
| Absorbance |
| Measurement range |
0 – 4 OD |
| OD accuracy |
0 – 2 OD: ≤ ± (1 % + 10 mOD) |
| OD reproducibility |
0 – 2 OD: ≤ ± (0.5 % + 5 mOD) |
| Fastest reading times (on-the-fly) |
| Fluorescence (kinetic cycle) |
1,536-well: 26 sec |
384-well: 19 sec |
| Fluorescence (plate in/out) |
1,536-well: 40 sec |
384-well: 35 sec |
| Injectors |
| Injection volume |
Variable; 1 µl increments |
| Syringe size |
Selectable: 500 / 1,000 / 2,500 µl |
| Pump speed |
100 – 300 µl/sec |
| Dead volume |
100 µl (including pump back) |
| Accessories |
| Stacker |
Connect |
| Barcode scanner |
left or right |
* 144-041 ATP detection kit SL (Biothema)
** ENLITEN® ATP assay system (Promega)
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