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Top class microplate reader offering outstanding performance for demanding applications in drug discovery and live sciences research.
The premium Quad4 Monochromators™ technology of the Infinite M1000 PRO plate reader offers total freedom of wavelength selection and instant access to new wavelengths as your assay requirements change.
The modular architecture is ideally suited to researchers as well as service providers, allowing customers to configure the instrument to their budget and detection demands.
Infinite® M1000 PRO - a selection of features at a glance
Premium Quad4 Monochromators™ technology for microplate readers |
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Tecan’s Quad4 Monochromators technology uses two excitation monochromators and two emission monochromators to offer outstanding flexibility, eliminating the cost and drawbacks of wavelength filters for research applications.
The premium Quad4 Monochromators system of the Infinite M1000 PRO plate reader further optimizes this technology, using a flash lamp with double the power and switchable flash frequency settings for fluorescence intensity to provide even more light to both the sample and the detector, as well as offering improved spectral blocking and extended stray light reduction down to 2x10-7. |
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The Quad4 Monochromators technology makes use of a double monochromator architecture on both the excitation (left) and emission sides (right). |
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Flexible wavelength selection and adjustable bandwidth settings |
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The Infinite M1000 PRO offers broad wavelength selection, from UV to NIR, with flexible bandwidth adjustment for optimization of fluorescence intensity based applications.
Patented technology allows the bandwidth of both the excitation and emission monochromators to be independently set in 1 nm steps. Bandwidth adjustments are possible for all fluorescence-based measurements, including fluorescence scans (excitation, emission and 3D), fluorescence polarization emission, FRET, time-resolved fluorescence (TRF) and time-resolved FRET (TR-FRET). Optimizing monochromator bandwidth results in improved instrument sensitivity, particularly for multiplexed and FRET assays. |
Infinite® M1000 PRO - Options
Modularity is the key to success! |
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| The Infinite M1000 PRO microplate reader is a modular multimode reader tailored for your specific needs; now and for the future. Experimental requirements in a laboratory can quickly change, and your equipment needs to be able to adapt just as quickly. Be prepared for the future with the modular design of the Infinite M1000 PRO plate reader. Whatever you measure, now or in the future, the fully upgradeable Infinite M1000 PRO is prepared. |
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Accessories |
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New injectors to save reagents!
The Infinite M1000 PRO has an optional, state-of-the-art injector module which uses up to two injectors to replace manual pipetting steps or trigger fast kinetic reactions in fluorescence, luminescence and absorbance modes. A tilting carrier and multiple syringe size options ensure low dead volumes to minimize wastage of substrates and buffers. The module offers adjustable injection volume and speed settings, features a bulk dispense function to eliminate tedious pipetting steps for 6- to 384-well plates, and has easily accessible prime/wash buttons to simplify injector maintenance. Detection of ultra-fast kinetics can also be achieved for a wide range of applications, by using the injector module in combination with the Infinite M1000 PRO’s well-wise measurement mode. |
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Optional injector module minimizes wastage of substrates, and is equipped
with metalfree needles for ion studies |
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Infinite® M1000 PRO - Applications
The Infinite M1000 PRO supports a broad range of microplate-based detection assays, please refer to the technical literature/download section for application notes, technical notes and posters.
- TR-FRET-based assays: all major formats e.g. HTRF® (Cisbio International, France), LanthaScreen® (Invitrogen).
- FRET-based assays: e.g. Z`-LYTE® [Invitrogen] to study cellular protein kinases, GeneBLAzer® [Invitrogen] Reporter Gene assays.
- Glow luminescence: cell-based & reporter gene assays
- Flash luminescence: e.g. dual luciferase assay (DLR®) and various Ca2+ detection assays
- AlphaScreen®/AlphaLISA®: homogenous bead based-assays
- Fluorescence polarization: e.g. Predictor™ hERG Fluorescence Polarization Assay [Invitrogen], PolarScreen™ Glucocorticoid Receptor Competitor Assay Kit, Red [Invitrogen], PolarScreen™ Glucocorticoid Receptor Competitor Assay Kit, Green [Invitrogen], Transcreener® ADP Assay [BellBrook Labs]
- Fast wavelength scanning: extremely useful when working with permanently changing and new fluorophores or with unknown spectra (resulting e.g. from unknown compounds). The Infinite M1000 PRO can rapidly perform 3D scans.
The Infinite M1000 PRO has been validated by Invitrogen for the following Invitrogen assays:
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The Infinite M1000 PRO has been granted the LanthaScreen® Certified Plus Status by Invitrogen
PolarScreen and Predictor are trademarks of Invitrogen Corporation, Carlsbad, USA.
LanthaScreen, Adapta, GeneBLAzer, Omnia and Z’-LYTE are registered trademarks of Invitrogen Corporation, Carlsbad, USA. |
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The Infinite M1000 PRO has been successfully validated and certified for HTRF® in white plates by CisBio bioassays.
HTRF® is a registered trademark of Cisbio international, France. The fluorescence ratio associated with the HTRF readout is a correction method developed by Cisbio international and covered by the US patent 5,527,684 and its foreign equivalents, for which Cisbio international has granted a license to Tecan Group Ltd. Its application is strictly limited to the use of HTRF reagents and technology, excluding any other TRFRET technologies. |
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The Infinite M1000 PRO has been successfully validated for Transcreener® Far Red FP and Transcreener® FI validated by BellBrook Labs, LLC, USA and meets the requirements for BellBrook Labs’ Instrument Validation Program.
Transcreener® HTS Assay Platform is a patented technology of BellBrook Labs, LLC USA. Transcreener® is a registered trademark of BellBrokk Labs, LLC USA. |
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The Infinite M1000 PRO has passed the DLReady™ validation at Promega and is certified as DLReady™.
DLReady™ is a trademark of Promega Corporation, USA. |
Infinite® M1000 PRO - Typical performance values
| Light sources |
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High energy xenon flash lamp, light-emitting diodes |
| Wavelength selection |
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Premium Quad4 Monochromators |
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Absorbance |
Fluorescence |
| Bandwidth |
Fixed, 5 nm |
Adjustable ≤ 300 nm: 2.5 – 10 nm;
> 300 nm: 5 – 20 nm |
| Wavelength accuracy |
± 0.3 nm |
≤ 300 nm: ± 0.5 nm;
> 300 nm: ± 1 nm |
| Wavelength reproducibility |
± 0.3 nm |
≤ 300 nm: ± 0.5 nm;
> 300 nm: ± 1 nm |
| Wavelength range |
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Absorbance |
Fluorescence |
FP |
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230 – 1,000 nm |
Ex: 230 – 850 nm; Em: 280 – 850 nm |
Ex (LED):
470, 530, 590, 635 nm
Em (Mono):
280 – 850 nm |
| Detectors |
| Absorbance |
UV Silicon photodiode, 4-channel parallel reading |
| Fluorescence (incl. FP) |
Extended wavelength (UV and far red)
Low dark current PMT |
| Luminescence |
Photon counting low dark current PMT |
| Plate formats |
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6 – 1536-well plates, NanoQuant Plate |
| Temperature control |
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Ambient +4 to 42 °C |
| Shaking |
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Linear, orbital, double orbital with variable amplitudes |
| Fluorescence sensitivity values |
| Fluorescence top reading |
25 amol/well fluorescein
(Greiner® 384-well low volume black plate; 10 µl) |
| Fluorescence bottom reading |
0.6 fmol/well fluorescein
(Greiner 96-well SensoPlate™; 200 µl) |
| TRF |
1.5 amol/well europium
(Greiner 384-well low volume white plate; 10 µl) |
| FP |
< 2 mP standard deviation @ 1 nM fluorescein |
| Luminescence sensitivity values |
| Glow luminescence* |
225 amol/well ATP
(Greiner 384-well low volume white plate; 25 µl) |
| Flash luminescence** |
12 amol/well ATP
(Greiner 384-well white plate; 55 µl) |
| Absorbance |
| Measurement range |
0 – 4 OD |
| OD linearity |
0 – 3 OD: R2 ≥ 0.999 |
| OD accuracy |
0 – 3 OD: ≤ ± (1 % + 6 mOD) |
| OD reproducibility |
0 – 3 OD: ≤ ± (0.5 % + 5 mOD) |
| AlphaScreen |
| Detection limit |
≤ 100 amol/well bio-LCK-P***
≤ 2,5 ng/ml Omnibeads**** |
| Uniformity |
≤ 3% CV*** |
| Z' Value |
≥ 0,9*** |
| Fastest read time |
< 2min (384-well plate) |
| Fastest reading times – fixed wavelength (on-the-fly) |
| Absorbance |
1,536-well: 19 sec |
384-well: 15 sec |
96-well: 11 sec |
| Fluorescence |
1,536-well: 35 sec |
384-well: 24 sec |
96-well: 17 sec |
| FP |
1,536-well: 100 sec |
384-well: 42 sec |
96-well: 22 sec |
| Fastest reading times – scanning (450 – 550 nm, 5 nm step) |
| Absorbance |
96-well: 35 sec |
| Fluorescence |
96-well: 115 sec |
| Injectors |
| Injection volume |
Variable; 1 µl increments |
| Syringe size |
Selectable: 500, 1000, 2500 µl |
| Pump speed |
100 – 300 µl/sec |
| Dead volume |
100 µl including pump back |
| Accessories |
| Stacker |
built-in with up to 50 microplates |
| Barcode scanner |
left or right |
* 144-041 ATP detection kit SL (Biothema)
** ENLITEN® ATP assay system (Promega)
*** (P-Tyr-100 Assay Kit, PE # 6760620); (384-well small volume plates)
****(PE # 6760626); (384-well small volume plates)
Infinite® M1000 PRO - Software
| The Infinite M1000 PRO microplate reader can be operated by two Tecan software packages, i-control™ and Magellan™. |
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i-controli-control is the standard system control software for all Infinite series plate readers, offering a simple and flexible user interface. It allows the user to create application-oriented measurement scripts with any combination of processing steps, and data is exported to Microsoft Excel®-compatible file formats. |
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Workflow oriented reader sofware |
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i-control application tab for NanoQuant Plate™ measurements |
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New luminescence scan mode for the Infinite M1000 PRO plate reader |
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AlphaScreen®/AlphaLISA® in i-control and Magellan |
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The latest i-control and Magellan software packages available for the Infinite M1000 PRO – include new controls for AlphaScreen and AlphaLISA measurements.
The instrument is equipped with dedicated filters for AlphaScreen- and AlphaLISA-based assays, which can be easily selected from a dropdown list in the AlphaScreen/AlphaLISA measurement stripe. Excitation times from 10 ms and signal integration periods from 100 ms can be defined. Depending on the sample volume, a custom definable settle time can be used to minimize fluid swashing effects.
The temperature correction function can be used for both Alpha assay types by activating the corresponding checkbox in the measurement stripe.
The measurement results will be displayed accordingly, i.e. in a raw or temperature-corrected way, in the data output. In addition to the custom-definable settings in the AlphaScreen/AlphaLISA measurement stripe, i-control and Magellan also offer dedicated predefined measurement scripts, including optimized settings for both Alpha assay types and scientific descriptions of the assay principle. These scripts are accessible in the templates section of the software. |
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AlphaScreen measurement stripe in i-control |
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AlphaLISA measurement stripe in i-control |
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Magellan |
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| Magellan is Tecan’s universal data reduction and analysis software, offering powerful analytical capabilities, excellent data presentation and outstanding graphical flexibility. Its spectra calculation package includes background correction, curve smoothing, wavelength selection, peak identification and 3D scanning tools, making it the ideal accompaniment to the Infinite M1000 PRO. The wizard-guided user interface ensures convenient handling of dilution series, IC calculations and data import and export, simplifying data processing and analysis. In addition, a version of Magellan that helps to fulfill the requirements of FDA regulation 21 CFR part 11 is also available. |
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Magellan is an easy-to-use wizardbased data analysis software |
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3D scanning enables rapid characterization of unknown samples |
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